In Vitro and In Vivo Anticancer Activity of Ferula Gummosa Essential Oil Nanoemulsions (FGEO-NE) for the Colon Cancer Treatment

This survey was performed to aim of synthesize Nano emulsion from Ferula gummosa essential oil (FEGO-NE) and evaluate its anti-tumor effect. First, analyzed by GC–MS method, then the synthesized as O/W after characterization DLS, Zeta potential, AFM, FESEM TEM methods, toxicity evaluated MTT method. Then pro-apoptotic effects were qPCR (Caspase3, 9, Bax Bcl-2) method AO/PI staining. The cancer induction model used antitumor in Balb/C mice. anti-angiogenic antioxidant (VEGF, CAT SOD) results physicochemical studies showed formation droplet with dimensions 24.6 nm, dispersion index 0.41 zeta potential ? 28.5 mV a spherical morphology. at concentration 2.9 ?g/mL inhibited about 50% ht-29 cells, while up 4 no inhibitory effect on normal cells. Increase caspase 3, 9 decrease BCL-2 gene expression along increase apoptotic cells AP/PI staining confirmed apoptosis FEGO-NE. FEGO-NE an angiogenesis additive genes. In addition, reduction tumor volume (69.72% 14 days) samples treated confirmed. this study that various mechanisms are able inhibit have reducing induced tumors vivo model. These suggest FEGO-NEs suitable candidate for therapy studies.